Journal
JOURNAL OF LIPID RESEARCH
Volume 56, Issue 7, Pages 1363-1369Publisher
ELSEVIER
DOI: 10.1194/jlr.D059725
Keywords
inflammation; lipoprotein; lipid transfer protein; systemic inflammatory response syndrome; sepsis; human; mouse; diagnostic tool; mass spectrometry; liquid chromatography tandem mass spectrometry
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Funding
- INSERM (Institut National de la Sante Et de la Recherche Medicale)
- Regional Council of Bourgogne
- European Regional Development Fund
- University of Bourgogne
- Fondation de France
- Oseo/Banque Publique d'Investissement
- French government [ANR-11-LABX-0021-01-Lip-STIC LabEx]
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Quantitation of plasma lipopolysaccharides (LPSs) might be used to document Gram-negative bacterial infection. In the present work, LPS-derived 3-hydroxymyristate was extracted from plasma samples with an organic solvent, separated by reversed phase HPLC, and quantitated by MS/MS. This mass assay was combined with the limulus amebocyte lysate (LAL) bioassay to monitor neutralization of LPS activity in biological samples. The described HPLC/MS/MS method is a reliable, practical, accurate, and sensitive tool to quantitate LPS. The combination of the LAL and HPLC/MS/MS analyses provided new evidence for the intrinsic capacity of plasma lipoproteins and phospholipid transfer protein to neutralize the activity of LPS. In a subset of patients with systemic inflammatory response syndrome, with documented infection but with a negative plasma LAL test, significant amounts of LPS were measured by the HPLC/MS/MS method. Patients with the highest plasma LPS concentration were more severely ill. HPLC/MS/MS is a relevant method to quantitate endotoxin in a sample, to assess the efficacy of LPS neutralization, and to evaluate the proinflammatory potential of LPS in vivo.
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