Journal
JOURNAL OF LIPID RESEARCH
Volume 56, Issue 3, Pages 737-746Publisher
ELSEVIER
DOI: 10.1194/jlr.D050120
Keywords
low density lipoprotein particle number; cardiovascular risk; nuclear magnetic resonance; two-dimensional
Categories
Funding
- CIBER de Diabetes y Enfermedades Metabolicas, an initiative of ISCIII (Ministerio de Ciencia e Innovacion)
- FIS [PI081579]
- EU FP5 Program Quality of Life and Management of Living Resources, Key Action 1, Food, Nutrition, and Health [QLK1-CT-1999-00830]
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Determination of lipoprotein particle size and number using advanced lipoprotein tests (ALTs) is of particular importance to improve cardiovascular risk prediction. Here we present the Liposcale test, a novel ALT based on 2D diffusion-ordered H-1 NMR spectroscopy. Our method uses diffusion coefficients to provide a direct measure of the mean particle sizes and numbers. Using 177 plasma samples from healthy individuals and the concentration of ApoB and ApoA from isolated lipoprotein fractions, our test showed a stronger correlation between the NMR-derived lipoprotein particle numbers and apolipoprotein concentrations than the LipoProfile (R) test commercialized by Liposcience. We also converted LDL particle numbers to ApoB equivalents (milligrams per deciliter) and our test yielded similar values of LDL-ApoB to the LipoProfile (R) test (absolute mean bias of 8.5 and 7.4 mg/dl, respectively). In addition, our HDL particle number values were more concordant with the calibrated values determined recently using ion mobility. Finally, principal component analysis distinguished type 2 diabetic patients with and without atherogenic dyslipidemia (AD) on a second cohort of 307 subjects characterized using the Liposcale test (area under the curve = 0.88) and showed concordant relationships between variables explaining AD. Altogether, our method provides reproducible and reliable characterization of lipoprotein particles and it is applicable to pathological states such as AD.
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