Journal
CLINICAL CANCER RESEARCH
Volume 17, Issue 17, Pages 5725-5735Publisher
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/1078-0432.CCR-11-1261
Keywords
-
Categories
Funding
- Dutch Cancer Society [KUN2006-3699]
- EU [ENCITE HEALTH-F5-2008-201842, LSHC-CT-2006-518234, DC-THERA LSB-CT-2004-512074]
- Netherlands Organization for Scientific Research [NWO-Vidi-917.76.363, AGIKO-92003250]
- AGIKO RUNMC
- Vanderes Foundation
- NOTK foundation
- NWO Spinoza
Ask authors/readers for more resources
Purpose: It is unknown whether the route of administration influences dendritic cell (DC)-based immunotherapy. We compared the effect of intradermal versus intranodal administration of a DC vaccine on induction of immunologic responses in melanoma patients and examined whether concomitant administration of interleukin (IL)-2 increases the efficacy of the DC vaccine. Experimental Design: HLA-A2.1(+) melanoma patients scheduled for regional lymph node dissection were vaccinated four times biweekly via intradermal or intranodal injection with 12 x 10(6) to 17 x 10(6) mature DCs loaded with tyrosinase and gp100 peptides together with keyhole limpet hemocyanin (KLH). Half of the patients also received low-dose IL-2 (9 MIUdaily for 7 days starting 3 days after each vaccination). KLH-specific B- and T-cell responses were monitored in blood. gp100- and tyrosinasespecific T-cell responses were monitored in blood by tetramer analysis and in biopsies from delayed-type hypersensitivity (DTH) skin tests by tetramer and functional analyses with (51) Cr release assays or IFN gamma release, following coculture with peptide-pulsed T2 cells or gp100-or tyrosinase-expressing tumor cells. Results: In 19 of 43 vaccinated patients, functional tumor antigen-specific T cells could be detected. Although significantly more DCs migrated to adjacent lymph nodes upon intranodal vaccination, this was also highly variable with a complete absence of migration in 7 of 24 intranodally vaccinated patients. Intradermal vaccinations proved superior in inducing functional tumor antigen-specific T cells. Coadministration of IL-2 did not further augment the antigen-specific T-cell response but did result in higher regulatory T-cell frequencies. Conclusion: Intradermal vaccination resulted in superior antitumor T-cell induction when compared with intranodal vaccination. No advantage of additional IL-2 treatment could be shown. Clin Cancer Res; 17(17); 5725-35. (C)2011 AACR.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available