Journal
CLINICAL BIOCHEMISTRY
Volume 47, Issue 6, Pages 448-454Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.clinbiochem.2013.12.022
Keywords
Cholera; Ogawa; Monoclonal antibody; Rapid flow test; Lateral flow device
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Funding
- Artron BioResearch Inc.
- State High-Tech Development Plan (863 program) [2002AA215015]
- Chongqing Medical scientific research project [2009-2-200]
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Objectives: Cholera is an acute malignant infectious disease caused by the bacteria Vibrio cholerae leading to severe dehydrating diarrhea and vomiting, even high rates of mortality in some cases. However, the prevention of the epidemic disease is achievable if proper sanitation practices are followed, provided the accurate and prompt diagnosis of each prevalent serotype in cholera epidemic. The current gold standard of bacterial culture is inadequate for rapid diagnosis. Our aim is to develop an immunochromatographic test format for O1 serotype Ogawa diagnosis and provide the need for better epidemic prevention and early response. Design and methods: The monoclonal antibodies were raised in conventional method and subsequently screened for a match pair. A variety of related and unrelated bacteria strains recruited were employed to test their sensitivity, specificity etc. by indirect ELISA. The human fecal samples were used to test the final lateral-flow device product to satisfy the measurement requirement. Results: A new monoclonal antibody (McAb) pair, named IXiao(3)G(6) and IXiao(1)D(9), was generated, which is specifically against V. cholerae O1 serotype Ogawa. Additionally, we developed an immunochromatographic lateral flow device (LFD) using this McAb pair for the highly specific and rapid (5 min) detection of Ogawa. Conclusions: Our product has advantages of simplicity and precision, and can benefit the scene and elementary medical institutions. (C) 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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