Association of the TNF-alpha-308G/A polymorphism with family history of type 2 diabetes mellitus in a Mexican population

Aims: We examined the possible association of the -308G/A polymorphism of the TNF-alpha promoter gene in type 2 diabetes mellitus (DM2) patients and in non-diabetic subjects with and without family history of DM2. Methods: We studied 87 non-diabetic subjects without DM2 family history in at least one of two generations, 48 non-diabetic subjects with DM2 family history and 95 DM2 patients. Genotyping was carried out by PCR-RFLP. Results: The frequency of TNF-alpha -308G/A genotype was significantly lower in non-diabetic subjects without DM2 relatives (6%) as compared to DM2 patients (24%) (odds ratio (OR) = 5.24; 95% confidence interval (CI) = 1.9-15.8, p < 0.0005), but similar to non-diabetic subjects with DM2 relatives (29%) (OR = 0.77: CI = 0.3-1.7, p = 0.4). Logistic regression analysis showed the association of TNF-alpha -308G/A polymorphism with DM2 family history (OR = 5.80; CI = 1.77-18.98, p < 0.0003). Conclusions: Our results suggest that TNF-alpha -308G/A polymorphism is associated with DM2 family history and is a risk factor for DM2. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.