Journal
CLINICAL BIOCHEMISTRY
Volume 44, Issue 4, Pages 348-350Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.clinbiochem.2010.12.011
Keywords
Clinical laboratory techniques; Inflammation; Nitric oxide; Oxidative stress; Vasodilation
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Objective: We described an automated technique for measurement of serum nitrite/nitrate (NOx) using the Cobas Mira clinical chemistry analyzer. Design and methods: NOx was measured by the modified Griess method. Precision, accuracy, linearity, instrument carry-over and lower limit of quantitation (LLOQ) were assessed. Results: The automated technique for measurement of serum NOx was linear, precise, and accurate. It has a LLOQ of 2.0 mu mol/L. Conclusion: Serum NOx measured by the modified Griess method can be applied easily to the Cobas Mira clinical chemistry analyzer. (C) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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