Journal
CLINICAL BIOCHEMISTRY
Volume 44, Issue 17-18, Pages 1445-1450Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.clinbiochem.2011.09.010
Keywords
Newborn screening; Disorder markers; Dried-blood spots; Storage conditions; Temperature effects; Humidity effects; Sample stability
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Funding
- Centers for Disease Control and Prevention
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Objective: We aimed to measure separately the contributions of heat and humidity to changes in levels of 34 markers of inborn disorders in dried-blood-spot (DBS) samples. Design and methods: We stored paired sets of DBSs at 37 degrees C for predetermined intervals in low-humidity and high-humidity environments. Marker levels of all samples in each complete sample set were measured in a single analytic run. Results: During the 30 +/- 5 day studies, galactose-1-phosphate uridyltransferase and biotinidase lost almost 65% of initial activities in low-humidity storage: most of the degradation in 27 other markers was attributable to adverse effects of high-humidity storage: seven markers in DBSs stored at high humidity lost more than 90% of initial levels by the end of the study and 4 of the 7 lost more than 50% of initial levels within the first week of storage. Conclusions: Minimizing both humidity and temperature in DES transportation and storage environments is essential to maintaining sample integrity. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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