Journal
CLINICAL BIOCHEMISTRY
Volume 42, Issue 1-2, Pages 91-98Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.clinbiochem.2008.09.120
Keywords
Diagnostic accuracy; Lactonase; Liver diseases; Paraoxonase; Reference interval
Categories
Funding
- Fondo de Investigacion Sanitaria [00/0232, 02/0430, 05/1607]
- Instituto de Salud Carlos III [C03/02, C03/08, 603/015]
- Spanish National Institute for Bioinformatics
- Generalitat de Catalunya [FI 05/00068]
- ICREA Funding Source: Custom
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Aims: We investigated the analytical performance of a new assay of the lactonase activity of paraoxonase-1 and its efficacy in the assessment of liver damage. Design and methods: Serum lactonase activity was determined by the hydrolysis of 5-thiobutyl butyrolactone in 633 healthy individuals and 369 patients with chronic liver disease. Paraoxonase-1, 2, and 3 gene polymorphisms were analyzed by the MassArray (TM) method. Results: Linearity was up to 10 U/L. Detection limit was 0.12 U/L. Imprecision was <= 17.7%. Lactonase values in our normal population were 5.99 (3.29-13.61) U/L. Lactonase activity showed a lower influence of genetic polymorphisms than the classical assay using paraoxon. Both measurements showed a similar efficiency in testing for liver dysfunction. Conclusion: We report a reliable assay using a non-toxic substrate for the measurement of serum lactonase activity. The influence of genetic variability is low. The assay could be a useful addition to tests evaluating liver impairment. (C) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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