Journal
CLINICAL BIOCHEMISTRY
Volume 42, Issue 6, Pages 540-543Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.clinbiochem.2008.12.026
Keywords
Nucleosides; Breast cancer; DNA damage; Liquid chromatography-mass spectrometry
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Funding
- Korean Institute of Science and Technology (KIST)
- Korean Ministry of Education, Science and Technology (MEST)
- Korean Science and Engineering Foundation (KOSEF)
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Objective: Evaluation of altered urinary nucleosides before and after tumor removal of breast cancer (BCa). Design and methods: Targeted metabolite profiling of 14 urinary nucleosides was conducted with both pre- and post-operative female patients with BCa (n = 150, age: 46.6 +/- 7.7 years), and female controls (n = 150, age: 46.8 +/- 7.7 years) by liquid chromatography-tandem mass spectrometry coupled to on-line extraction. Results: Levels of modified nucleosides (5-hydroxymethyl-2'-deoxyuridine, P<0.001 8-hydroxy-2'-deoxyguanosine, P<0.001; 1-methyladenosine, P<0.02; N-2,N-2-dimethylguanosine, P<0.001) were significantly higher in pre-operative patients than in both normal controls and post-operative patients. Conclusions: This approach could be used to further understand the pathogenesis of BCa as well as to evaluate the effects of medical treatment. (C) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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