Journal
CLINICAL AND VACCINE IMMUNOLOGY
Volume 18, Issue 8, Pages 1343-1350Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/CVI.05104-11
Keywords
-
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology [21022034, 21249028, 21406010]
- Ministry of Health, Labor, and Welfare, Japan [H20-Shinkou-ippan-013, H21-Chikyukibo-ippan-005]
- Bill and Melinda Gates Foundation
- Grants-in-Aid for Scientific Research [21249028, 21406010, 23406007] Funding Source: KAKEN
Ask authors/readers for more resources
The aim of a malaria transmission-blocking vaccine is to block the development of malaria parasites in the mosquito and thus prevent subsequent infection of the human host. Previous studies have demonstrated that the gametocyte/gamete surface protein Pfs230 can induce transmission-blocking immunity and have evaluated Escherichia coli-produced Pfs230 as a transmission-blocking vaccine candidate. In this study, we used the wheat germ cell-free expression system to produce N-terminal fragments of Pfs230 and evaluated the transmission-blocking activity of antisera raised against the recombinant Pfs230 protein. The rabbit antisera reacted to the surface of cultured gametocytes and gametes of the Plasmodium falciparum NF54 line, recognized the 360-kDa form of parasite-produced Pfs230 by Western blot assay, and reduced the infectivity of NF54 parasites to Anopheles stefensi mosquitoes in the presence of complement in a standard membrane feeding assay. Thus, our data demonstrate that the N-terminal pro domain of Pfs230 is sufficient to induce complement-dependent transmission-blocking activity against P. falciparum.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available