Journal
CLINICAL AND VACCINE IMMUNOLOGY
Volume 17, Issue 3, Pages 384-392Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/CVI.00503-09
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Funding
- National Institutes of Health (NIH)
- National Institute of Allergy and Infectious Diseases (NIAID) [AI-067665, T32 AI-07501, AI-07289, AI-033774, AI-052733, HL-059842, AI-033146, AI-056257]
- Center for AIDS Research [AI-51519]
- Institute for Clinical and Translational Research [UL1 RR-025750, KL2 RR-025749, TL1 RR-025748]
- Albert Einstein College of Medicine and Montefiore Medical Center
- Tuberculosis Research Materials and Vaccine Testing Contract at Colorado State University [AI-40091]
- General Clinical Research Center at the New York University School of Medicine [M01 RR-00096]
- Fogarty Institute Center [TW01409]
- Department of Veterans Affairs
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The immunodominance of Mycobacterium tuberculosis proteins malate synthase (MS) and MPT51 has been demonstrated in case-control studies with patients from countries in which tuberculosis ( TB) is endemic. The value of these antigens for the serodiagnosis of TB now is evaluated in a cross-sectional study of pulmonary TB suspects in the United States diagnosed to have TB, HIV-associated TB, or other respiratory diseases (ORD). Serum antibody reactivity to recombinant purified MS and MPT51 was determined by enzyme-linked immunosorbent assays (ELISAs) of samples from TB suspects and well-characterized control groups. TB suspects were diagnosed with TB (n = 87; 49% sputum microscopy negative, 20% HIV+) or ORD (n = 63; 58% HIV+). Antibody reactivity to MS and MPT51 was significantly higher in U. S. HIV+/TB samples than in HIV+/TB samples (P < 0.001), and it was significantly higher in both TB groups than in control groups with latent TB infection ( P < 0.001). Antibody reactivity to both antigens was higher in U. S. HIV+/TB samples than in HIV+/ORD samples (P = 0.052 for MS, P = 0.001 for MPT51) but not significantly different between HIV+/TB and HIV+/ORD. Among U. S. HIV+ TB suspects, a positive anti-MPT51 antibody response was strongly and significantly associated with TB ( odds ratio, 11.0; 95% confidence interval, 2.3 to 51.2; P = 0.002). These findings have implications for the adjunctive use of TB serodiagnosis with these antigens in HIV+ subjects.
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