Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 54, Issue 35, Pages 10216-10219Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201503215
Keywords
bioorthogonal chemistry; chemical biology; proteins; single-molecule imaging; super-resolution microscopy
Categories
Funding
- DFG [SPP 1623, GRK 1986]
- Cluster of Excellence Macromolecular Complexes
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Protein labeling with synthetic fluorescent probes is a key technology in chemical biology and biomedical research. A sensitive and efficient modular labeling approach (SLAP) was developed on the basis of a synthetic small-molecule recognition unit (Ni-trisNTA) and the genetically encoded minimal protein His(6-10)-tag. High-density protein tracing by SLAP was demonstrated. This technique allows super-resolution fluorescence imaging and fulfills the necessary sampling criteria for single-molecule localization-based imaging techniques. It avoids masking by large probes, for example, antibodies, and supplies sensitive, precise, and robust size analysis of protein clusters (nanodomains).
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