Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 55, Issue 4, Pages 1531-1534Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201508414
Keywords
enzymes; host-guest systems; nanoreactors; protein engineering; synthetic biology
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Funding
- ETH Zurich
- European Research Council [ERC-dG-2012-321295]
- Uehara Memorial Foundation
- ETH Zurich - Marie Curie Actions program
- Stipendienfonds der Schweizerischen Chemischen Industrie (SSCI)
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Genetic fusion of cargo proteins to a positively supercharged variant of green fluorescent protein enables their quantitative encapsulation by engineered lumazine synthase capsids possessing a negatively charged lumenal surface. This simple tagging system provides a robust and versatile means of creating hierarchically ordered protein assemblies for use as nanoreactors. The generality of the encapsulation strategy and its effect on enzyme function were investigated with eight structurally and mechanistically distinct catalysts.
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