Journal
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
Volume 163, Issue 1, Pages 33-49Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2249.2010.04272.x
Keywords
ELISPOT; freezing; shipping; T cell; tetramer
Categories
Funding
- Juvenile Diabetes Research Foundation (JDRF) [5-2009-413]
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P>Autoimmune T cell responses directed against insulin-producing beta cells are central to the pathogenesis of type 1 diabetes (T1D). Detection of such responses is therefore critical to provide novel biomarkers for T1D 'immune staging' and to understand the mechanisms underlying the disease. While different T cell assays are being developed for these purposes, it is important to optimize and standardize methods for processing human blood samples for these assays. To this end, we review data relevant to critical parameters in peripheral blood mononuclear cell (PBMC) isolation, (cryo)preservation, distribution and usage for detecting antigen-specific T cell responses. Based on these data, we propose recommendations on processing blood samples for T cell assays and identify gaps in knowledge that need to be addressed. These recommendations may be relevant not only for the analysis of T cell responses in autoimmune disease, but also in cancer and infectious disease, particularly in the context of clinical trials.
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