Journal
CLINICAL & EXPERIMENTAL METASTASIS
Volume 25, Issue 6, Pages 611-619Publisher
SPRINGER
DOI: 10.1007/s10585-008-9186-y
Keywords
DT; AT3; dunning; FGFR2; alternative splicing; EMT; MET; imaging
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Funding
- NCI NIH HHS [R33 CA97502, R33 CA097502] Funding Source: Medline
- NIGMS NIH HHS [R01 GM063090] Funding Source: Medline
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Using alternative splicing reporters we have previously observed mesenchymal epithelial transitions in Dunning AT3 rat prostate tumors. We demonstrate here that the Dunning DT and AT3 cells, which express epithelial and mesenchymal markers, respectively, represent an excellent model to study epithelial transitions since these cells recapitulate gene expression profiles observed during human prostate cancer progression. In this manuscript we also present the development of two new tools to study the epithelial transitions by imaging alternative splicing decisions: a bichromatic fluorescence reporter to evaluate epithelial transitions in culture and in vivo, and a luciferase reporter to visualize the distribution of mesenchymal epithelial transitions in vivo.
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