Journal
CLINICAL & EXPERIMENTAL METASTASIS
Volume 25, Issue 3, Pages 241-252Publisher
SPRINGER
DOI: 10.1007/s10585-007-9138-y
Keywords
B16F10 melanoma cells; MDA-MB-231 breast cancer cells; laminin-111; bone metastasis; AG73; fibronectin
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Laminin-111 promotes the malignant phenotype, and a 12-mer synthetic peptide (AG73, RKRLQVQLSIRT) from the carboxyl terminus of the alpha 1 chain increases B16F10 melanoma metastasis to the lung and liver. Using an antibody array, fibronectin was identified as an up-regulated protein in B16F10 cells after incubation with this peptide. The increased fibronectin is cell-associated with no increase in soluble fibronectin. The AG73 peptide increased the number and size of bone metastases with both B16F10 melanoma and MDA-231 breast carcinoma cells in an intracardiac injection model. Using siRNA transfection, we found that a reduction in fibronectin expression did not reduce bone metastasis in the presence of the metastasis-promoting peptide AG73. We conclude that the laminin peptide AG73 increases metastasis independently of fibronectin expression.
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