Journal
CLINICA CHIMICA ACTA
Volume 418, Issue -, Pages 97-101Publisher
ELSEVIER
DOI: 10.1016/j.cca.2012.12.032
Keywords
Intestinal permeability; Lactulose-to-mannitol ratio; Sucralose; Sucrose; Mass spectrometry; Multiple reaction monitoring
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Funding
- NINR's Intramural Research Training Awards
- [1ZIANR000018-03]
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Background: Abnormal gastrointestinal permeability has been linked to irritable bowel syndrome (IBS). The lactulose-to-mannitol ratio is traditionally used to assess small intestine permeability while sucralose and sucrose are used to assess colonic and gastric permeability respectively. We used a single 4-probe test solution to assess permeability throughout the gastrointestinal tract in IBS patients and healthy controls by measuring the recovery of the probes in urine after ingestion using a modified liquid chromatography mass spectrometry protocol. Methods: Fasting participants (N=59) drank a permeability test solution (100 ml: sucralose, sucrose, mannitol, and lactulose). Urine was collected over a 5-h period and kept frozen until analysis. Urinary sugar concentrations were measured using a liquid chromatography/triple quadruple mass spectrometer. Results: Colonic permeability was significantly lower in IBS patients when compared to healthy controls (p=0.011). Gastric and small intestinal permeability did not significantly differ between the groups. Conclusions: The study demonstrates the clinical potential of this non-invasive method for assessing alterations in gastrointestinal permeability in patients with IBS. Published by Elsevier B.V.
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