Journal
CLINICA CHIMICA ACTA
Volume 420, Issue -, Pages 121-127Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.cca.2012.10.039
Keywords
MALDI-TOF/TOF MS; Citrulline; Quantification; Graphitized carbon nanoparticles; Parallel fragmentation monitoring
Categories
Funding
- Direct Grant for Research Fund from the Chinese University of Hong Kong
- Li Ka Shing Foundation
Ask authors/readers for more resources
Background: Selected reaction monitoring (SRM) is a reliable mass spectrometry (MS)-based technique for quantification of small molecules. However, it is not applicable to matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem MS (MALDI-TOF/TOF MS) instruments. This work presents a novel comparable MALDI-TOF/TOF MS technique, Parallel Fragmentation Monitoring (PFM), for high-throughput quantification of citrulline. Method: Calibrator/sample solutions were spiked with internal standard that was a stable isotopic analog with 1 mass unit heavier than citrulline. Both citrulline and internal standard were isolated and fragmented in parallel by MALDI-TOF/TOF MS in the presence of graphitized carbon nanoparticles as matrix. The ratio of the peak intensities of the selected fragment of citrulline to that of internal standard was used to calibrate/calculate the concentrations of citrulline in samples. Results: Linear calibration curves were obtained in the range of 10-250 mu mol/l citrulline with correlation coefficients >= 0.997. Stored calibration curve and batch-specific calibration curve produced highly similar measurement values. Within- and between-day CVs were 3.1-8.7% and 3.5-10.6%, respectively, illustrating the reliability and robustness of PFM. Conclusion: Using citrulline for proof-of-concept, we have developed the PFM technique with tremendous potential for high-throughput quantification of amino acids and other small molecules. (c) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available