Journal
CLINICA CHIMICA ACTA
Volume 419, Issue -, Pages 122-126Publisher
ELSEVIER
DOI: 10.1016/j.cca.2013.02.008
Keywords
Uremic toxin; Renal failure; Haemodialysis; Chromatography; Biological variation; Reference range
Categories
Ask authors/readers for more resources
Introduction: The uremic toxins indoxyl sulphate (IS) and p-cresyl sulphate (pCS) are absorbed bacterial metabolites of tryptophan and tyrosine respectively and may be predictive of clinical outcome. Long chromatography times, incomplete data on the reference ranges of the free and total fractions and the biological variation limit wider clinical application. Methods: An UPLC method with fluorescence detection was developed and reference ranges and biological variation were investigated in healthy volunteers. Results: Chromatography time was 3 min with excellent linearity, precision and low detection limits (IS of 0.02 mu mol/L and pCS of 0.05 mu mol/L). Both IS and pCS increased with a decrease in renal function and were moderately correlated with eGFR (R-2 0.65 and 0.33 respectively). The serum reference ranges were (mu mol/L): total IS of 0.7-63; free IS of 0.0-02; total pCS of 0.0-38.4; and free pCS of 0.1-2.4. The intra individual biological variation was estimated at 35.9% and 50.5% with a critical difference of 3.9 mu mol/L (100%) and 20.7 mu mol/L (141%) for total IS and pCS respectively. Conclusion: We describe a robust analytical method with a short chromatography time that quantifies both IS and pCS. The data on reference ranges and intra-individual biological variation need to be considered in clinical studies that investigate these uremic toxins. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available