Journal
CLINICA CHIMICA ACTA
Volume 413, Issue 7-8, Pages 719-726Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.cca.2012.01.004
Keywords
PR3-ANCA; MPO-ANCA; ELISA; Wegener's granulomatosis; Microscopic polyangiitis; GPA; MPA
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Background: The detection of anti-proteinase 3 (PR3) and anti-myeloperoxidase (MPO) autoantibodies represents a serological hallmark in the diagnosis of small vessel vasculitis such as granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA). We evaluated novel chemiluminescence assays (CIAs) for PR3- and MPO-ANCA detection and investigated their utility for disease activity monitoring. Methods: Sera collected from CPA (n = 41) and MPA (n = 30) patients were tested by QUANTA Lite (R) PR-3 and MPO ELISAs (INOVA Diagnostics) and by the QUANTA Flash (TM) PR3 and MPO CIAs (INOVA). Precision and linearity were analyzed following reference guidelines. The recently launched reference sera for PR3-and MPO-ANCA (Centers of Disease Control and prevention, CDC) were used to establish international units for the new assays. Disease activity was determined using the Birmingham Vasculitis Activity Score. Results: The international standards for PR3-and MPO-ANCA yielded results of 403 CU and 332 CU in the novel CIAs, respectively. The linearity analysis showed linear regression values >0.97 with slopes between 0.96 and 1.04. Total variation obtained from the precision study showed CV% of <= 7.4 for PR3-ANCA and <= 12.8 for MPO-ANCA. Good agreement (Spearman rho >= 0.89) was observed between CIA and ELISA. PR3-ANCA determined by CIA, but not by ELISA, was correlated with disease activity. No correlation was found for MPO-ANCA. Conclusion: The novel PR3- and MPO-ANCA CIAs show good precision, linearity and correlation to ELISA. In addition, PR3-ANCA by CIA show correlation with disease activity. (C) 2012 Elsevier B.V. All rights reserved.
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