Journal
CLINICA CHIMICA ACTA
Volume 406, Issue 1-2, Pages 45-51Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.cca.2009.05.008
Keywords
CST1; Cystatin SN; Cathepsin; Proliferation; Gastric cancer
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Funding
- Ministry of Science & Technology in Korea [FG09-12-01]
- KRIBB Research Initiative Program
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Background: Cysteine proteases like cathepsins are widely distributed proteolytic enzymes and form tight equimolar complexes with cystatins at their active sites. Among cystatins, CST1, encoding cystatin SN, is a member of the type 2 salivary cystatin family found in a variety of fluids and secretions, including plasma, tears, and saliva. CST1 was identified as an upregulated gene in gastric cancer tissues compared to noncancerous regions using our Affymetrix GeneChip microarray. Methods: The upregulation of CST1 in gastric cancer was analyzed using RT-PCR (n = 15). immnohistochemistry, and clinicopathological (n = 77) analysis. CST1-siRNA was used for the suppression of CST1 gene expression and cathepsin proteolytic activity was assayed. Results: CST1 was upregulated in cancerous lesions of gastric cancer tissues compared to noncancerous regions and clinicopathological analysis showed a significant correlation between high expression of CST1 and pTNM stage (p = 0.044). In CST1-siRNA transfected cells, cell proliferation was reduced and the proteolytic activity of cathepsins was increased. Conclusions: CST1 might be highly involved in gastric tumorigenesis and regulate the proteolytic activity of cysteine proteases. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.
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