Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 54, Issue 40, Pages 11652-11656Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201501777
Keywords
click chemistry; controlled release; DNA nanotechnology; fluorescence; FRET
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Funding
- Advanced ERC EU Award (NanoSensoMach)
- FET Open EU program
- MULTI project
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DNAzyme-capped mesoporous SiO2 nanoparticles (MP SiO2 NPs) are applied as stimuli-responsive containers for programmed synthesis. Three types of MP SiO2 NPs are prepared by loading the NPs with Cy3-DBCO (DBCO = dibenzocyclooctyl), Cy5-N-3, and Cy7-N-3, and capping the NP containers with the Mg2+, Zn2+, and histidine-dependent DNAzyme sequences, respectively. In the presence of Mg2+ and Zn2+ ions as triggers, the respective DNAzyme-capped NPs are unlocked, leading to the click reaction product Cy3-Cy5. In turn, in the presence of Mg2+ ions and histidine as triggers the second set of DNAzyme-capped NPs is unlocked leading to the Cy3-Cy7 conjugated product. The unloading of the respective NPs and the time-dependent formation of the products are followed by fluorescence spectroscopy (FRET). A detailed kinetic model for the formation of the different products is formulated and it correlates nicely with the experimental results.
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