Journal
CIRCULATION-HEART FAILURE
Volume 5, Issue 3, Pages 357-365Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/CIRCHEARTFAILURE.111.964692
Keywords
beta(2)-adrenergic receptors; transverse tubules; excitation-contraction coupling; heart failure; SERCA2a gene therapy; remodeling heart failure
Categories
Funding
- Fondation Leducq
- Wellcome Trust
- British Heart Foundation [FS/11/67/28954, NH/10/3/28574]
- Biotechnology and Biological Sciences Research Council [BB/E52708X/1] Funding Source: researchfish
- British Heart Foundation [NH/10/3/28574, SP/09/007/27920, RG/10/11/28457, FS/11/67/28954] Funding Source: researchfish
- Medical Research Council [G0500373] Funding Source: researchfish
- BBSRC [BB/E52708X/1] Funding Source: UKRI
- MRC [G0500373] Funding Source: UKRI
Ask authors/readers for more resources
Background-Cardiomyocyte surface morphology and T-tubular structure are significantly disrupted in chronic heart failure, with important functional sequelae, including redistribution of sarcolemmal beta(2)-adrenergic receptors (beta(2)AR) and localized secondary messenger signaling. Plasticity of these changes in the reverse remodeled failing ventricle is unknown. We used AAV9.SERCA2a gene therapy to rescue failing rat hearts and measured z-groove index, T-tubule density, and compartmentalized beta(2)AR-mediated cAMP signals, using a combined nanoscale scanning ion conductance microscopy-Forster resonance energy transfer technique. Methods and Results-Cardiomyocyte surface morphology, quantified by z-groove index and T-tubule density, was normalized in reverse-remodeled hearts after SERCA2a gene therapy. Recovery of sarcolemmal microstructure correlated with functional beta(2)AR redistribution back into the z-groove and T-tubular network, whereas minimal cAMP responses were initiated after local beta(2)AR stimulation of crest membrane, as observed in failing cardiomyocytes. Improvement of beta(2)AR localization was associated with recovery of beta AR-stimulated contractile responses in rescued cardiomyocytes. Retubulation was associated with reduced spatial heterogeneity of electrically stimulated calcium transients and recovery of myocardial BIN-1 and TCAP protein expression but not junctophilin-2. Conclusions-In summary, abnormalities of sarcolemmal structure in heart failure show plasticity with reappearance of z-grooves and T-tubules in reverse-remodeled hearts. Recovery of surface topology is necessary for normalization of beta(2)AR location and signaling responses. (Circ Heart Fail. 2012;5:357-365.)
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available