Journal
CIRCULATION RESEARCH
Volume 110, Issue 4, Pages 536-U84Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/CIRCRESAHA.111.254730
Keywords
MAP kinase pathway; ubiquitin; diabetes mellitus; myocardial infarction; apoptosis
Funding
- Mochida Memorial Foundation for Medical and Pharmaceutical Research
- America Heart Association [SDG 0930360N, 0740013N, 0740021N]
- National Institute of Health [HL102746-03, HL088637, HL108551, HL077789, HL088400]
- British Heart Foundation [PG/09/052/27833] Funding Source: researchfish
- Worldwide Cancer Research [10-0134] Funding Source: researchfish
- Grants-in-Aid for Scientific Research [23790830] Funding Source: KAKEN
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Rationale: Cardiomyocyte apoptosis is one of the key events in the development and progression of heart failure, and a crucial role for ICER (inducible cAMP early repressor) in this process has been previously reported. ERK5 is known to inhibit cardiac apoptosis after myocardial infarction (MI), especially in hyperglycemic states, via association with CHIP ubiquitin (Ub) ligase and subsequent upregulation of CHIP ligase activity, which induces ICER ubiquitination and subsequent protein degradation. The regulatory mechanism governing ERK5/CHIP interaction is unknown. Objective: We previously demonstrated increased p90RSK activation in the diabetic heart. As a logical extension of this work, we now investigate whether p90RSK activation inhibits ERK5-mediated CHIP activation, and subsequently increases ICER levels and apoptosis. Methods and Results: p90RSK activation inhibits ERK5/CHIP association and CHIP Ub ligase activity. p90RSK and CHIP share a common binding site in the ERK5 C-terminal domain (aa571-807). Overexpression of either p90RSK or an ERK5 fragment (aa571-807) inhibits ERK5/CHIP association, suggesting that p90RSK and CHIP competes for ERK5 binding and that p90RSK activation is critical for inhibiting ERK5/CHIP interaction. We also identified ERK5-S496 as being directly phosphorylated by p90RSK and demonstrated that an ERK5-S496A mutant significantly impairs Angiotensin II-mediated inhibition of CHIP activity and subsequent increase in ICER levels. In vivo, either cardiac-specific depletion of ERK5 or overexpression of p90RSK inhibits CHIP activity and accelerates cardiac apoptosis after MI-a phenomenon fully reversible by activating ERK5. Conclusions: These data suggest a role for p90RSK in inhibiting CHIP activity and promoting cardiac apoptosis through binding to and phosphorylation of ERK5-S496. (Circ Res. 2012;110:536-550.)
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