Journal
CIRCULATION RESEARCH
Volume 103, Issue 8, Pages 864-U212Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/CIRCRESAHA.108.178517
Keywords
bestrophins; calcium-activated chloride channel; cGMP; posttranscriptional regulation; vascular biology
Funding
- Danish Research Council
- Danish Heart Foundation
- Swedish Research Council
- European Union Sixth Framework Programme(RIGHT program)
- Danish National Research Foundation (Danmarks Grundforskningsfond)
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Although the biophysical fingerprints (ion selectivity, voltage- dependence, kinetics, etc) of Ca2+-activated Cl- currents are well established, their molecular identity is still controversial. Several molecular candidates have been suggested; however, none of them has been fully accepted. We have recently characterized a cGMP- dependent Ca2+-activated Cl- current with unique characteristics in smooth muscle cells. This novel current has been shown to coexist with a classic (cGMP- independent) Ca2+-activated Cl- current and to have characteristics distinct from those previously known for Ca2+-activated Cl- currents. Here, we suggest that a bestrophin, a product of the Best gene family, is responsible for the cGMP- dependent Ca2+-activated Cl- current based on similarities between the membrane currents produced by heterologous expressions of bestrophins and the cGMP- dependent Ca2+-activated Cl- current. This is supported by similarities in the distribution pattern of the cGMP- dependent Ca2+-activated Cl- current and bestrophin-3 (the product of Best-3 gene) expression in different smooth muscle. Furthermore, downregulation of Best-3 gene expression with small interfering RNA both in cultured cells and in vascular smooth muscle cells in vivo was associated with a significant reduction of the cGMP- dependent Ca2+-activated Cl- current, whereas the magnitude of the classic Ca2+-activated Cl- current was not affected. The majority of previous suggestions that bestrophins are a new Cl- channel family were based on heterologous expression in cell culture studies. Our present results demonstrate that at least 1 family member, bestrophin-3, is essential for a well-defined endogenous Ca2+-activated Cl- current in smooth muscles in the intact vascular wall.
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