Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 54, Issue 8, Pages 2510-2514Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201410645
Keywords
cancer sensing; dyes; mitochondria; polarity; ratiometric imaging
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Funding
- NSF of China [21136002, 21376039, 21422601, 21421005]
- National Basic Research Program of China [2013CB733702]
- Ministry of Education [NCET-12-0080]
- Liaoning NSF [2013020115]
- Fundamental Research Funds for the Central Universities [DUT14ZD214]
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Mitochondrial polarity strongly influences the intracellular transportation of proteins and interactions between biomacromolecules. The first fluorescent probe capable of the ratiometric imaging of mitochondrial polarity is reported. The probe, termed BOB, has two absorption maxima ((abs)=426 and 561nm) and two emission maximaa strong green emission ((em)=467nm) and a weak red emission (642nm in methanol)when excited at 405nm. However, only the green emission is markedly sensitive to polarity changes, thus providing a ratiometric fluorescence response with a good linear relationship in both extensive and narrow ranges of solution polarity. BOB possesses high specificity to mitochondria (R-r=0.96) that is independent of the mitochondrial membrane potential. The mitochondrial polarity in cancer cells was found to be lower than that of normal cells by ratiometric fluorescence imaging with BOB. The difference in mitochondrial polarity might be used to distinguish cancer cells from normal cells.
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