Journal
CIRCULATION
Volume 130, Issue 5, Pages 419-430Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/CIRCULATIONAHA.113.008364
Keywords
fibroblasts; fibrosis; glycogen synthase kinase 3 beta; hypertrophy; myocardial infarction
Funding
- National Heart, Lung, and Blood Institute [HL061688, HL091799]
- Canadian Institutes of Health Research [FRN 12858]
- American Heart Association Scientist Development Grant [13SDG16930103]
Ask authors/readers for more resources
Background-Myocardial infarction-induced remodeling includes chamber dilatation, contractile dysfunction, and fibrosis. Of these, fibrosis is the least understood. After myocardial infarction, activated cardiac fibroblasts deposit extracellular matrix. Current therapies to prevent fibrosis are inadequate, and new molecular targets are needed. Methods and Results-Herein we report that glycogen synthase kinase-3 beta (GSK-3 beta) is phosphorylated (inhibited) in fibrotic tissues from ischemic human and mouse heart. Using 2 fibroblast-specific GSK-3 beta knockout mouse models, we show that deletion of GSK-3 beta in cardiac fibroblasts leads to fibrogenesis, left ventricular dysfunction, and excessive scarring in the ischemic heart. Deletion of GSK-3 beta induces a profibrotic myofibroblast phenotype in isolated cardiac fibroblasts, in post-myocardial infarction hearts, and in mouse embryonic fibroblasts deleted for GSK-3 beta. Mechanistically, GSK-3 beta inhibits profibrotic transforming growth factor-beta 1/SMAD-3 signaling via interactions with SMAD-3. Moreover, deletion of GSK-3 beta resulted in the significant increase of SMAD-3 transcriptional activity. This pathway is central to the pathology because a small-molecule inhibitor of SMAD-3 largely prevented fibrosis and limited left ventricular remodeling. Conclusions-These studies support targeting GSK-3 beta in myocardial fibrotic disorders and establish critical roles of cardiac fibroblasts in remodeling and ventricular dysfunction.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available