Journal
CIRCULATION
Volume 124, Issue 4, Pages 454-U269Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/CIRCULATIONAHA.111.032268
Keywords
atherosclerosis; apolipoproteins E; low-density lipoprotein receptor-related protein 1; Ly6-C antigen, mouse; monocytes; apoptosis
Funding
- National Institutes of Health [HL57986, HL086988]
- Atherosclerosis Core of Vanderbilt Mouse Metabolic Phenotyping Centers (National Institutes of Health) [DK59637]
- Office Of The Director
- EPSCoR [903795] Funding Source: National Science Foundation
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Background-We previously demonstrated that macrophage low-density lipoprotein receptor (LDLR)-related protein 1 (LRP1) deficiency increases atherosclerosis despite antiatherogenic changes including decreased uptake of remnants and increased secretion of apolipoprotein E (apoE). Thus, our objective was to determine whether the atheroprotective effects of LRP1 require interaction with apoE, one of its ligands with multiple beneficial effects. Methods and Results-We examined atherosclerosis development in mice with specific deletion of macrophage LRP1 (apoE(-/-) M Phi LRP1(-/-)) and in LDLR-/- mice reconstituted with apoE(-/-) M Phi LRP1(-/-) bone marrow. The combined absence of apoE and LRP1 promoted atherogenesis more than did macrophage apoE deletion alone in both apoE-producing LDLR-/- mice (+88%) and apoE(-/-) mice (+163%). The lesions of both mouse models with apoE(-/-) LRP1(-/-) macrophages had increased macrophage content. In vitro, apoE and LRP1 additively inhibit macrophage apoptosis. Furthermore, there was excessive accumulation of apoptotic cells in lesions of both LDLR-/- mice (+110%) and apoE(-/-) M Phi LRP1(-/-) mice (+252%). The apoptotic cell accumulation was partially due to decreased efferocytosis as the ratio of free to cell-associated apoptotic nuclei was 3.5-fold higher in lesions of apoE(-/-) M Phi LRP1(-/-) versus apoE(-/-) mice. Lesion necrosis was also increased (6 fold) in apoE(-/-) M Phi LRP1(-/-) versus apoE(-/-) mice. Compared with apoE(-/-) mice, the spleens of apoE(-/-) M Phi LRP1(-/-) mice contained 1.6- and 2.4-fold more total and Ly6-C-high monocytes. Finally, there were 3.6- and 2.4-fold increases in Ly6-C-high and CC-chemokine receptor 2-positive cells in lesions of apoE(-/-) M Phi LRP1(-/-) versus apoE(-/-) mice, suggesting that accumulation of apoptotic cells enhances lesion development and macrophage content by promoting the recruitment of inflammatory monocytes. Conclusion-Low-density lipoprotein receptor protein 1 exerts antiatherogenic effects via pathways independent of apoE involving macrophage apoptosis and monocyte recruitment. (Circulation. 2011; 124: 454-464.)
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