Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 54, Issue 18, Pages 5317-5322Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201412406
Keywords
compartmentalization; encapsulation; FRET; polymersomes; protein modifications
Categories
Funding
- Australian Research Council (ARC) centre of excellence [CE140100036]
- ARC [FT120100101]
- Faculty of Science UNSW
- Australian Government
- ARC
- UNSW
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Polymersomes provide a good platform for targeted drug delivery and the creation of complex (bio) catalytically active systems for research in synthetic biology. To realize these applications requires both spatial control over the encapsulation components in these polymersomes and a means to report where the components are in the polymersomes. To address these twin challenges, we synthesized the protein-polymer bioconjugate PNIPAM-b-amilFP497 composed of thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) and a green-fluorescent protein variant (amilFP497). Above 37 degrees C, this bioconjugate forms polymersomes that can (co) encapsulate the fluorescent drug doxorubicin and the fluorescent light-harvesting protein phycoerythrin 545 (PE545). Using fluorescence lifetime imaging microscopy and Forster resonance energy transfer (FLIM-FRET), we can distinguish the co-encapsulated PE545 protein inside the polymersome membrane while doxorubicin is found both in the polymersome core and membrane.
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