Journal
JOURNAL OF INORGANIC BIOCHEMISTRY
Volume 153, Issue -, Pages 284-292Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2015.10.014
Keywords
Drug delivery; Calcium carbonate nanoparticles; Layer-by-layer; Cisplatin
Funding
- University of Salento - Bando 5 PER MILLE PER LA RICERCA 2012 - Project NanoDEPT [PON 254/Ric.]
- Potenziamento del CENTRO RICERCHE PER LA SALUTE DELL'UOMO E DELL'AMBIENTE [PONa3_00334]
- PRIN [1152/ric]
- NANOMED [prot 2010FPTBSH, CUP: F81J12000380001]
- [CUP: F81D11000210007]
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A smart nanocarrier system for cancer therapy, based on a recently developed technique for preparing pure nanometric calcium carbonate (CaCO3), was studied. Different approaches were used to obtain sustained release of cisplatin: at first, pure CaCO3 nanoparticles were evaluated as carriers, then the nanoparticles were functionalized with polymer or silanes, and finally they were employed as a substrate to build layer by layer (LbL) self-assembled polyelectrolyte nanocapsules. Loading efficiency and release kinetics were measured. The best loadings were obtained with the LbL nanocapsules, allowing for high loading efficiency and the possibility of controlling the release rate of the drug. The behavior of all the carriers was evaluated on four neoplastic cell lines, representative of different types of neoplastic disease, namely MCF-7 (breast cancer), SKOV-3 (ovarian cancer), HeLa (cervical cancer) and CACO-2 (human epithelial colorectal adenocarcinoma). Negligible cytotoxicity of the nanoparticles, functionalized nanoparticles, and nanocapsules was observed in experiments with all cell lines. Nanocapsules were functionalized with fluorescein isothiocyanate (FTTC) in order to track their kinetic of internalization and localization in the cell line by confocal laser scanning microscopy (CLSM). The cytotoxicity of the loaded capsules was evaluated, showing cell survival rates close to those expected for non-encapsulated cisplatin at the same nominal concentration. (C) 2015 Elsevier Inc. All rights reserved.
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