Journal
CHROMATOGRAPHIA
Volume 81, Issue 11, Pages 1467-1474Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10337-018-3601-6
Keywords
Caffeine; Histidine; Polymer modification; Solid-phase extraction; Tea
Funding
- University of Innsbruck
- Medical University of Innsbruck
- Austrian Academy of Sciences at the Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck
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This work presents the synthesis of a polymeric mixed-mode solid-phase extraction (SPE) sorbent for clean-up and isolation of caffeine from black and green tea samples. The material was synthesized by a simple thermallyinitiated copolymerization of glycidyl methacrylate and ethylene glycol dimethacrylate. Further functionalization was executed with histidine (HIS). Functional groups were investigated by attenuated total-reflection infrared spectroscopy. Furthermore, nitrogen sorption porosimetry was executed and revealed surface areas of 90m(2)g(-1). Adsorption capacities for caffeine were compared between functionalized and non-modified polymers and showed maximum capacities of 3.01 and 4.82mgg(-1) polymer, respectively. Time adsorption profiles revealed an equilibrium adsorption after 15min. The proposed polymer was used for SPE of black and green tea extracts and showed excellent clean-up efficiency for isolation of caffeine with recoveries ranging from 89 to 93%. When compared to commercially available Oasis HLB, the HIS-functionalized polymer demonstrated a distinctly better performance for clean-up. Finally, the proposed method was validated regarding international (ICH) guidelines and regulations.
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