Journal
CHROMATOGRAPHIA
Volume 75, Issue 17-18, Pages 1009-1016Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10337-012-2199-3
Keywords
Capillary electrophoresis; Carbohydrates; Maple syrup; Maple sugar; 1-Phenyl-3-methyl-5-pyrazolone
Ask authors/readers for more resources
Reducing carbohydrates in maple syrup and maple sugar were separated by capillary electrophoresis using derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) and the characteristics of these samples were studied. Reducing carbohydrate standards including nine monosaccharides and five disaccharides as PMP derivatives could be easily resolved by using 200 mM borate buffer (pH 10.5) as a background electrolyte. Glucose was the most abundant reducing sugar in both maple samples, and mannose was abundant relative to the other sugars. The other monosaccharides (xylose, arabinose, ribose, galactose and N-acetylglucosamine) were also detected. When maple syrup and maple sugar were treated with invertase, which removed fructose residues from the reducing ends of fructosyl saccharides, melibiose was detected, suggesting that raffinose exists in both samples. The differences of carbohydrate contents between maple syrup and maple sugar were also discussed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available