Journal
CHROMATOGRAPHIA
Volume 73, Issue 7-8, Pages 673-680Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10337-011-1926-5
Keywords
Cell membrane chromatography; Gas chromatography-mass spectrometry; Methyl nonyl ketone; Peritoneal macrophage; Anti-inflammatory
Funding
- Xi'an Jiaotong University
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The aim of this study was to screen for the anti-inflammatory components from Houttuynia cordata Thunb (HCT) using an analytical method combining cell membrane chromatography (CMC) with gas chromatography-mass spectrometry (GC-MS). The stationary phase of the CMC employed mouse peritoneal macrophage (PM) cell membranes. We investigated the performance of the PM/CMC-offline-GC-MS method using hydrocortisone (HC) as standard. The method was then applied to the identification of anti-inflammatory components in extracts of HCT. The major component retained by CMC was identified as methyl nonyl ketone (MNK) by GC-MS. In vitro experiments revealed that MNK was able to inhibit LPS-induction of TNF-alpha, NO, and H2O2 production in a dose-dependent manner. IC50 values were 3.3, 4.1, and 3.6 mu g mL(-1), respectively. The PM/CMC-offline-GC-MS method is an effective screening system for the rapid detection, enrichment, and identification of target components from complex samples.
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