Journal
CHROMATOGRAPHIA
Volume 71, Issue 9-10, Pages 941-946Publisher
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-010-1551-8
Keywords
Column liquid chromatography; Solid lipid nanoparticles; Ketoconazole, ritonavir and lopinavir
Funding
- CSIR, New Delhi
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Lopinavir (LPV), ritonavir (RTV) and ketoconazole (KTZ) loaded solid lipid nanoparticles (SLN) were formulated based on reported drug-drug interaction among these three drugs. In order to study the drug-drug interactions and to optimize the formulation, the in vitro release data of these three drugs from the formulation is necessary. Hence, there is a need to develop a simple, accurate and a rapid RP-LC method for the simultaneous determination of KTZ, RTV and LPV. The present method was developed using Kromasil C18 column (250 mm x 4.5 mm, 5 mu m) with mobile phase consisting of acetonitrile: 0.2% triethylamine pH adjusted to 6.5 (60:40 v/v) at a flow rate of 1 mL min(-1). Chromatographic separation was monitored at 210 nm. Retention times of KTZ, RTV and LPV were 6.24, 8.05 and 9.23 min respectively. The method was linear over range of 0.5-10 mu g mL(-1). This method was successfully applied for the determination of KTZ, RTV and LPV in SLN.
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