Journal
CHROMATOGRAPHIA
Volume 71, Issue 9-10, Pages 933-939Publisher
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-010-1535-8
Keywords
Column liquid chromatography-mass spectrometry; Arginine, ornithine and citrulline; Symmetric dimethylarginine and asymmetric dimethylarginine
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A rapid and specific liquid chromatography-mass spectrometry method was developed for the simultaneous determination of underivatized arginine, ornithine, citrulline, symmetric and asymmetric dimethylarginine in human plasma. The plasma samples were extracted with acetonitrile after addition of isotopic internal standards and then chromatographed on an Atlantis dC(18) column (4.6 x 100 mm, 3.0 mu m). The mobile phase was composed of 0.03% trifluoroacetic acid in water and the flow rate was 0.6 mL min(-1). Selected ion monitoring detection was performed on a single-quadrupole mass spectrometer using positive electrospray ionization. The method was well validated and then successfully applied to a clinical study in healthy subjects.
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