Journal
CHROMATOGRAPHIA
Volume 71, Issue 5-6, Pages 481-485Publisher
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-009-1457-5
Keywords
High-speed counter-current chromatography; Phenylpropanoids; Daphnetin and woonenoside XI; Daphne giraldii
Funding
- Bureau of Science & Technology of Dalian City [2008J23JH042]
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Preparative high-speed counter-current chromatography (HSCCC) was successfully applied to purify phenylpropanoids from the stem and root bark of Daphne giraldii Nitsche, a traditional Chinese medicine. Their structures were identified on the basis of H-1 NMR and C-13 NMR technology. The two-phase solvent system composed of n -hexane-ethyl acetate-methanol-water (2: 3: 0.5: 4, v/v/v/v) was selected for HSCCC. A total of 8.0 mg woonenoside XI (1) and 18.0 mg daphnetin (2) were obtained in one-step separation from 200 mg of the crude extract with purity of 96.0 and 99.1%, respectively, as determined by LC. And the major compound (2) showed antithrombotic activity in vitro.
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