Journal
CHROMATOGRAPHIA
Volume 72, Issue 3-4, Pages 337-341Publisher
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-010-1648-0
Keywords
Capillary electrophoresis; Chemiluminescence; Pharmacokinetics; Norfloxacin and ciprofloxacin in urine
Funding
- National Natural Science Foundation of China [20575056]
- Henan Innovation Project for University Research Talents [2005126]
- Natural Science Foundation of Henan Province of China [092300410122]
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A highly sensitive and rapid method for the determination of norfloxacin and ciprofloxacin was developed using capillary electrophoresis coupled with chemiluminescence detection. The chemiluminescence detection was based on the enhancing effects of two analytes on the luminol-hydrogen peroxide reaction. The baseline separation of norfloxacin and ciprofloxacin was obtained within 4.5 min under the optimum conditions. The limits of detection (S/N = 3) of norfloxacin and ciprofloxacin were 2.9 x 10(-10) and 1.1 x 10(-12) mol L(-1), respectively. The method showed wide linear range with four orders of magnitude (R a parts per thousand yen 0.9982). The limits of quantification (S/N = 10) of two analytes in human urine samples were 6.0 x 10(-8) and 5.1 x 10(-10) mol L(-1), respectively. The proposed method was successfully applied to the analysis of norfloxacin and ciprofloxacin in human urine samples and the monitoring of pharmacokinetics of ciprofloxacin in human body.
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