Journal
CHROMATOGRAPHIA
Volume 70, Issue 3-4, Pages 637-641Publisher
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-009-1191-z
Keywords
Gas chromatography-electron capture detection; Solid phase extraction; Human plasma; N-Heptafluorobutyryl heptafluorobutyl ester; 3-Nitrotyrosine
Funding
- Italian Ministry for University and Research
- General Management for Strategies
- Development of Internationalisation of Scientific and Technological Research
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A method suitable for the determination of 3-nitrotyrosine, a well known marker of nitro-oxidative stress, in plasma by GC-ECD has been investigated. After deproteinisation, SPE and LC separation, 3-nitrotyrosine was derivatised by a single-step procedure to the corresponding N-heptafluorobutyryl heptafluorobutyl ester and analysed. The precision and reliability of the procedure were assured by using 4-nitrophenylalanine as internal standard and a diverter valve to protect the detector. The method was applied to the analysis of volunteers' plasma samples.
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