Flavone Glycoside Based Validated RP-LC Method for Quality Evaluation of Prishniparni (Uraria picta)

An LC method is developed for the quantitation of rhoifolin in Uraria picta, a plant of high usage frequency in all Asian traditional systems of medicine. An isocratic RP-LC method using C-18 column, UV detection 265 nm and specificity with PDA and MS is speeding up, reliable and comprehensive analysis of rhoifolin in U. picta. Good linearity was obtained in the working range (0.02-0.10 mg mL(-1)), with correlation coefficients > 0.99. LOD and LOQ were 2.33 and 7.69 ng, respectively. The method was validated following international guidelines. The described method can be utilized for assays and stability tests of U. picta extracts as well as Ayurvedic drugs based on Prishniparni.