Journal
CHROMATOGRAPHIA
Volume 70, Issue 11-12, Pages 1703-1708Publisher
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-009-1361-z
Keywords
Column liquid chromatography; Protein precipitation; Bupropion and hydroxybupropion in human plasma
Funding
- Research Council of Anadolu University [060324]
- GlaxoSmithKline (Istanbul, Turkey)
- Scientific and Technical Research Council of Turkey (TUBITAK)
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A new LC method has been developed and validated for the direct determination of bupropion and its main metabolite, hydroxybupropion in human plasma. Plasma samples were analyzed after a simple, one step protein precipitation with trichloroacetic acid using a C-8 column and mobile phase, consisting of methanol/acetonitrile/phosphate buffer (10 mM, pH 3.0) (40:10:50, v/v/v) and 20 mM 1-heptane sulfonic acid sodium salt with carbamazepine as the internal standard. UV detection was performed at 214 and 254 nm. The method was validated over the concentration range of 60-2,400 and 150-4,700 ng mL(-1) for bupropion and hydroxybupropion, respectively. The intra- and inter-day assay variability was less than 15% for the two analytes. Limit of detection values were 24.8 and 63.4 ng mL(-1) for bupropion and hydroxybupropion, respectively. The method developed was applied to quantification of bupropion and hydroxybupropion in human plasma.
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