Journal
CHROMATOGRAPHIA
Volume 70, Issue 7-8, Pages 1291-1293Publisher
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-009-1287-5
Keywords
Capillary electrophoresis-electrochemiluminescence detection; Liquid-liquid extraction; Enantioseparation; Dioxopromethazine hydrochloride
Funding
- National Natural Science Foundation of China [20605020]
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Capillary electrophoresis coupled with electrochemiluminescence detection was developed for the separation and determination of dioxopromethazine hydrochloride (DPZ) enantiomers. Performance parameters of the proposed method were evaluated. An improved separation of DPZ enantiomers could be achieved after adding boric acid to buffer. The enantiomers were completely separated with running buffer of 16.5 mM beta-CD in 25 mM tris-H3PO4-40 mM H3BO3 at pH 2.5. The proposed method was successfully applied to the separation and determination of DPZ enantiomers in human urine with a liquid-liquid extraction procedure.
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