Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 43, Issue 1, Pages 45-53Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-015-1707-5
Keywords
Glutathione; Escherichia coli; Bifunctional glutathione synthetase; One-strain system; Energy regeneration
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Funding
- National Science Foundation for Young Scientist of China [21406065]
- Fundamental Research Funds for the Central Universities [222201313007, 22A201514042]
- National Special Fund for State Key Laboratory of Bioreactor Engineering [2060204]
- Shanghai Committee of Science and Technology [13DZ1930202]
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Glutathione (GSH) is an important bioactive substance applied widely in pharmaceutical and food industries. Due to the strong product inhibition in the GSH biosynthetic pathway, high levels of intracellular content, yield and productivity of GSH are difficult to achieve. Recently, a novel bifunctional GSH synthetase was identified to be less sensitive to GSH. A recombinant Escherichia coli strain expressing gshF encoding the bifunctional glutathione synthetase of Streptococcus thermophilus was constructed for GSH production. In this study, efficient GSH production using this engineered strain was investigated. The cultivation process was optimized by controlling dissolved oxygen (DO), amino acid addition and glucose feeding. 36.8 mM (11.3 g/L) GSH were formed at a productivity of 2.06 mM/h when the amino acid precursors (75 mM each) were added and glucose was supplied as the sole carbon and energy source.
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