Journal
JOURNAL OF IMMUNOLOGY
Volume 195, Issue 4, Pages 1883-1890Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1402103
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Funding
- Keio University Global Center of Excellence Program
- Leading Project for Biosimulation
- Japan Society for the Promotion of Science [25430139]
- Ministry of Education, Culture, Sport, Science and Technology, Japan
- Grants-in-Aid for Scientific Research [25430139, 23249030, 26111003] Funding Source: KAKEN
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Immunodeficient hosts exhibit high acceptance of xenogeneic or neoplastic cells mainly due to lack of adaptive immunity, although it still remains to be elucidated how innate response affects the engraftment. IL-2R common gamma-chain (IL-2R gamma(c)) signaling is required for development of NK cells and a subset of dendritic cells producing IFN-gamma. To better understand innate response in the absence of adaptive immunity, we examined amounts of metastatic foci in the livers after intrasplenic transfer of human colon cancer HCT116 cells into NOD/SCID versus NOD/SCID/IL-2R gamma(c) null ( NOG) hosts. The intravital microscopic imaging of livers in the hosts depleted of NK cells and/or macrophages revealed that IL-2R gamma(c) function critically contributes to elimination of cancer cells without the need for NK cells and macrophages. In the absence of IL-2R gamma(c), macrophages play a role in the defense against tumors despite the NOD Sirpa allele, which allows human CD47 to bind to the encoded signal regulatory protein alpha to inhibit macrophage phagocytosis of human cells. Analogous experiments using human pancreas cancer MIA PaCa-2 cells provided findings roughly similar to those from the experiments using HCT116 cells except for lack of suppression of metastases by macrophages in NOG hosts. Administration of mouse IFN-gamma to NOG hosts appeared to partially compensate lack of IL-2R gamma(c)-dependent elimination of transferred HCT116 cells. These results provide insights into the nature of innate response in the absence of adaptive immunity, aiding in developing tumor xenograft models in experimental oncology.
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