Poly(N-vinylpyrrolidone)-modified surfaces repel plasma protein adsorption

The present work aimed to study the interaction between plasma proteins and PVP-modified surfaces under more complex protein conditions. In the competitive adsorption of fibrinogen (Fg) and human serum albumin (HSA), the modified surfaces showed preferential adsorption of HSA. In 100% plasma, the amount of Fg adsorbed onto PVP-modified surfaces was as low as 10 ng/cm(2), suggesting the excellent protein resistance properties of the modified surfaces. In addition, immunoblots of proteins eluted from the modified surfaces after plasma contact confirmed that PVP-modified surfaces can repel most plasma proteins, especially proteins that play important roles in the process of blood coagulation.