Journal
CHINESE JOURNAL OF PHYSIOLOGY
Volume 56, Issue 5, Pages 298-305Publisher
NATL DEFENSE MEDICAL CENTER
DOI: 10.4077/CJP.2013.BAB186
Keywords
3,5,3 '-triiodothyronine; apoptosis; FSH; granulosa cell; proliferation
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Funding
- Innovation Team on Nutrition & Feedstuff in Poultry, Beijing, PRC [PXM2012_014207_0001666]
- Technology Foundation for Selected Overseas Chinese Scholar, Beijing Municipal Bureau of Personnel, Beijing, PRC
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Thyroid hormone (TH) is important for normal reproductive functions and dysregulation of TH support is associated with reproductive disorders. We have previously reported that 3,5,3'-triiodothyronine (T-3) increases follicle stimulating hormone (FSH)-induced preantral follicle growth in vitro. Interaction of hormones with apoptosis and proliferation of granulosa cells is poorly understood. The present study investigated the role and the mechanism of T-3 and/or FSH on granulosa cell apoptosis and proliferation. Granulosa cells harvested from DES-primed immature rats were exposed to T-3 (1 nM) and/or FSH (100 ng/ml) for 24-48 h. We demonstrated by TUNEL assays that the hormones prevented cells from C8-ceramide-induced apoptosis. The Src/PI3K/Akt pathway was involved in the regulation of granulosa cell survival. While ineffective alone, T-3 significantly enhanced the proliferating cell nuclear antigen (PCNA) content of FSH-induced granulosa cells, consistent with the cell number pattern after treatment. Moreover, the action of the hormones on cell proliferation was also shown to be mediated by the Src/PI3K/Akt pathway. Taken together, these results suggest that T-3 potentiates the cell survival action of FSH through inhibiting cell apoptosis and promoting cell proliferation. Moreover, the protective and survival effects of hormones are mediated by the activation of Src/PI3K/Akt pathway.
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