Journal
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
Volume 29, Issue 2, Pages 290-296Publisher
SCIENCE PRESS
DOI: 10.1007/s00343-011-0048-0
Keywords
microalgae; Nannochloropsis oculata; EPA; delta-6 desaturase; gene expression
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Funding
- National Key Technology R&D Program of China [2006BAD09A03-2]
- National High-tech Research and Development Program of China (863 Program) [2007AA09Z427]
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A gene (NANOC-D6D) encoding a desaturase that removes two hydrogen atoms from fatty acids at delta 6 position was isolated from a cDNA library of Nannochloropsis oculata (Droop) D. J. Hibberd (Eustigmatophyceae). The unicellular marine microalga N. oculata synthesizes rich long chain polyunsaturated fatty acids (LCPUFAs), including eicosapentaenoic acid (20:5n-3, EPA). The deduced protein contains 474 amino acids that fold into 4 trans-membrane domains. The neighbor-joining phylogenetic tree indicates that NANOC-D6D is phylogenetically close to the delta-6 fatty acid desaturase of marine microalgae such as Glossomastix chrysopiasta, Thalassiosira pseudonana, and Phaeodactylum tricornutum. The gene was expressed in Saccharomyces cerevisiae INVSc1 to verify the substrate specificity of NANOC-D6D. Our results suggest that the recombinant NANOC-D6D simultaneously desaturates linoleic acid (LA) and alpha-linolenic acid (ALA).
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