Journal
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
Volume 19, Issue 6, Pages 424-431Publisher
SPRINGER
DOI: 10.1007/s11655-011-0856-2
Keywords
apoptosis; cardiomyocytes; berberine; norepinephrine
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Funding
- National Natural Science Foundation of China [30670826, 30971191]
- Science and Technology Foundation from Ministry of Education of the People's Republic of China [207140]
- Guangdong Science and Technology Projects [2008B030301352]
- Fundamental Research Funds for the Central Universities [21609405]
- Leading Academic Discipline Program, 211 Project for Jinan University
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To determine the effect of berberine (Ber) on norepinephrine (NE)-induced apoptosis in neonatal rat cardiomyocytes. The cultured neonatal rat cardiomyocytes were treated with NE in the presence or absence of Ber. The activity of lactate dehydrogenase (LDH) in the culture medium was examined, and apoptosis of cardiomyocytes was assessed by Hoechst 33258, isothiocyanate (FITC)-conjugated annexin-V, and propidine iodide (PI) staining. In addition, the activities of caspases-2 and-3 were measured by a fluorescent assay kit. The level of secreted tumor necrosis factor alpha (TNF-alpha) and production of intracellular reactive oxygen species (ROS) were also determined. NE at a concentration of 50 mu mol/L induced an obvious increase in the activity of LDH in the culture medium (P < 0.05), which was inhibited by coincubation with 0.5, 1.0, or 2.0 mu mol/L Ber (P < 0.05). Ber also significantly attenuated NE-induced apoptosis in a dose-dependent manner (P < 0.01). Moreover, Ber at a dose of 2 mu mol/L markedly decreased the ROS and TNF-alpha productions (P < 0.05) and inhibited the activation of caspases-2 and -3 in cardiomyocytes exposed to NE (P < 0.05)h. The present study suggested that Ber could reduce NE-induced apoptosis in neonatal rat cardiomyocytes through inhibiting the ROS-TNF-alpha-caspase signaling pathway.
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