Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 421, Issue -, Pages 61-72Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2015.02.003
Keywords
Citrobacter rodentium; Mucosal model of inflammation; Infection-induced colitis
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Funding
- Crohn and Colitis Foundation of America SRA [294083]
- Russian Scientific Fund [14-50-00060]
- Russian Science Foundation [14-50-00060] Funding Source: Russian Science Foundation
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Citrobacter rodentium is a natural mouse pathogen which reproducibly infects mice and causes intestinal disease. The C. rodentium model of infection is very useful for investigating host pathogen immune interactions in the gut, and can also be used to understand the pathogenesis of several important human intestinal disorders, including Crohn's disease, ulcerative colitis, dysbiosis and colon tumorigenesis. Both innate and adaptive immune responses play a critical role in protection against C. rodentium. Here, we summarize the role of immune components in protection against C. rodentium and describe techniques for the analysis of innate and adaptive mucosal immune responses, including setting up the infection, analysis of colonic hyperplasia and bacterial dissemination, evaluation of antibody responses, and purification and analysis of intestinal epithelial and lymphoid cells. (C) 2015 Elsevier B.V. All rights reserved.
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