Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 421, Issue -, Pages 89-95Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2015.03.014
Keywords
T cells; Intraepithelial lymphocytes; Intestinal epithelial cells; 3-D cultures
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Funding
- Crohn's & Colitis Foundation of America Senior Research Award
- National Institutes of Health [NIH R01 DK093674]
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The constant interaction between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) is thought to regulate mucosal barrier function and immune responses against invading pathogens. IELs represent a heterogeneous population of mostly activated and antigen-experienced T cells, but the biological function of IELs and their relationship with IECs is still poorly understood. Here, we describe a method to study T-cell-epithelial cell interactions using a recently established long-term intestinal enteroid culture system. This system allowed the study of peripheral T cell survival, proliferation, differentiation and behavior during long-term co-cultures with crypt-derived 3-D enteroids. Peripheral T cells activated in the presence of enteroids acquire several features of IELs, including morphology, membrane markers and movement in the epithelial layer. This co-culture system may facilitate the investigation of complex interactions between intestinal epithelial cells and immune cells, particularly allowing long term-cultures and studies targeting specific pathways in IEC or immune cell compartments. (C) 2015 Elsevier B.V. All rights reserved.
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