Journal
CHINESE JOURNAL OF CHEMISTRY
Volume 29, Issue 5, Pages 1031-1035Publisher
WILEY-BLACKWELL
DOI: 10.1002/cjoc.201190175
Keywords
biosensor; mercury; graphene; fluorescence
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Funding
- National 973 Project [2009CB930601]
- National Natural Science Foundation of China [20874048, 20903057, 21005040]
- Natural Science Foundation of Jiangsu Province [BK2008452]
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A highly sensitive and selective, turn-on and simple Hg2+ biosensor is reported by using water-soluble graphene oxide (WSGO) and dye-labeled mercury(II)-specific oligonucleotide (MSO) probe. The probe is rich of thymine (T) and can readily form the stem-loop structure which consists of the T-Hg2+-T configuration. In the absence of Hg2+, the probe exists as a random coil conformation which can be readily adsorbed on the surface of WSGO by strong noncovalent binding of bases, as a result, the fluorescence of the dye labeled on the terminus of the MSO is strongly quenched by the efficient electron/energy transfer from the dye to WSGO. Upon addition of Hg2+, the formation of the T-Hg2+-T structure releases the MSO from the surface of WSGO, resulting in a restoration of the fluorescence of dye-labeled MSO probe. Based on this observation, a highly sensitive and selective Hg2+ sensor is developed, which can work with turn-on mode in aqueous solutions at room temperature. By using the fluorometric method, the limit of detection for Hg2+ can reach picomolar range (187 pmol.L-1), and it is demonstrated that the biosensor is highly selective and only minimally perturbed by a wide range of non-specific metal ions.
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